Diy mycology lab

DIYbioDo It Yourself biology is a growing movement that aims to make biology accessible outside of professional contexts. Over the past few years, platforms such as OpenPCR and Pearl Biotech transilluminator have been designed to support biology work in schools and maker spaces for a fraction of the cost of professional lab equipment.

These exciting developments pave the way for crowdsourcing biology research amongst students, hobbyists, and non-experts. Our project contributes to the growing number of open source biology tools by developing a low-cost, yet relatively precise and easy-to-use incubator.

An incubator is an essential tool for a number of biological experiments and is often used in bacterial cell culture experiments.

Keeping It Clean: How to Design and Build a Laminar Flow Hood

Given the higher price range of the commercially available incubators upwards of several thousand dollarsnon-professional biology enthusiasts might not afford to add an incubator to their stock of DIYbio tools. The goal of the incubator is to keep a constant temperature inside. On the most basic level, we are using a temperature sensor and a light bulb as our heating element. The change in temperature inside the incubator is proportional to the amount of heat emitted by the tungsten bulb minus the heat loss through the walls of the incubator.

In other words, to increase the temperature, the bulb needs to emit more heat than the heat loss through the walls. The amount of heat emitted by the tungsten bulb is proportional to the power applied to the bulb. Hence, we can control the temperature inside the incubator by controlling the AC input supplied to the bulb.

Did you use this instructable in your classroom? Add a Teacher Note to share how you incorporated it into your lesson. In this step, we discuss how to control the AC supply using AC phase control mechanism. This step has some technical details before we get into the practical steps of assembling the incubator. In AC phase control, we control the input power supplied to the load in our case the tungsten bulb by allowing only a part of each half cycle of the AC signal to go through it.

Unlike DC supplies, where the voltage is constant at any given time, the voltage of an AC supply follows a sine waveform. Therefore we need to know the current position of the AC cycle exactly before allowing it to go through the load in order to achieve desired output power.

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Simply, the power control mechanism should be synchronised with the input AC signal. For that we use a Zero-Crossing detector.

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As the name suggests, zero-crossing point is where the AC signal crosses the 0V level or the point it changes its direction. This happens at the beginning and the end of each half cycle and the zero crossing detector outputs a square pulse at each of these points.

Once we detect this point we can wait T1 amount of time before we switch ON the AC signal to go through the load. By controlling the T1 we can control the output power of the load.Click any link below and If you've done any checking into hoods, you know most places charge an arm and a leg for them. These plans are for a 12"x24" Flow Hood like the one pictured below.

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The two largest costs are the fan blower and the HEPA filter. If you can get a hold of a used squirrel cage style blower that would be perfect. These are used in furnaces and industrial applications mostly. If you know anyone in HVAC they can get you one for practically nothing. Otherwise, a good place is HERE. You will need at least a cfm blower to power a 12"x24" HEPA filter figuring.

diy mycology lab

Skill saw or table saw jig saw even, anything to make a straight cut. If you have a Lowe's, Menard, Home Depot or any other large home store, check there. Most of these places have pre-cut wood in their lumber sections that work perfect for this project with very minimal cutting. If you have access to a saw, then it's way cheaper to buy a large sheet and cut it yourself.

To get started, you need to cut all the pieces for the box itself. When you have these pcs.

diy mycology lab

It helps to have someone to hold pcs. Drill pilot holes for all screws so as not to split the wood. Put together sides, top and bottom first. Don't tighten the screws all the way tight, you may need some room to slide the back in. When the sides, top and bottom are together, slide the back in either end, it doesn't matter yet.

Before you screw the back in, tighten the rest of the screws holding the box together. Make sure it's lined up flush and screw it in place. The purpose of the 1x1's is to create a flange 6" deep to push the filter against so a tight seal is created. Once the box is complete and the flange is in, it's time to cut a hole in the top for the blower to push air through.

Measure the hole in your blower, and draw it out on the top of your hood about 1" from the back a bit more if your blower needs. Center it from side to side. Then cut this out. Once the hole for the blower is cut, you need to clean out any sawdust real well.During these uncertain times we understand that there may be some confusion about which stores will be open and which stores will not.

As a purveyor of both agricultural and laboratory equipment Myco Supply is considered an essential business and will remain open. Please rest assured that Myco Supply will continue to serve our customers throughout this critical time. Many are using our synthetic filter discs in conjunction with a face mask to help prevent the spread of and in an effort to help fight against Covid Our discs are made from a virgin polypropelene PP plastic which can be steam sterilized or autoclaved in order to be reused.

They are highly durable and have a filtration rate of Sadly, we do not sell masks or the material to make the mask itself. We can only offer the protective filter. In regards to making a mask, please use the pattern on this link and follow the steps shown here:.

Myco Supply offers mushroom growing supplies, tools and equipment for building your own mushroom growing kit, and mycology supplies and equipment. We also offer fungi growing products for edible mushroom growers and fungi enthusiasts! Curious about mushroom cultivation or in creating your own mushroom grow kits?

Whether you are just starting out growing and looking to build your own mushroom growing kit, or you are a seasoned professional looking to replenish your mushroom supply, Myco Supply has all the growing supplies needed to get the job done right.

From rye berries, coconut coir bricks, cow manure and other mushroom substrate to mushroom growing bags, plastic jar lids, synthetic filter discs, and other mushroom equipment, all the mushroom grow tools and mycology equipment you need are available here at Myco Supply.

Mushrooms and other fungi grow best when the correct tools and nutrients are used.

I Am Building The Ultimate Lab For My Mushroom Farm - Triple Flow Hood Workbench Plenum

Vermiculite for Mushrooms — Professional-Grade. Miniature Bales of Shredded Wheat Straw. Rye Grass Seed.

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Spawn Bags with Filter Patch. Synthetic Filter Discs.One equipment I wanted most but beyond my reach was a Laminar Flow Hood. A Laminar Flow Hood provides a small sterile work area, where you can carry out all your culture works without the risk of contamination, simply by flowing completely purified air at a specified rate.

However, the cost of the simplest form of the equipment is very prohibitive The alternative? Construct your own equipment In a horizontal type, the purified air is blown from the back and moves towards the front of the working area.

This Instructable will provide you step-by-step instructions on how to build your own portable Laminar Flow Hood without any compromise on standard specifications. Did you use this instructable in your classroom? Add a Teacher Note to share how you incorporated it into your lesson. I could lay my hands on a slightly larger sized HEPA filter used in window air conditioners. I have removed it from the original frame, reduced it to 29 centimeters by 15 centimeters size and mounted the filter to frame work made of Plexiglas.

This frame work is further fixed to the center of another frame of size 40 centimeters by 25 centimeters by cutting out the center portion to the required size. This frame is also made from Plexiglas. You can see the entire assembly of the HEPA filter in the first picture. Plexiglas comes with protective covering on both sides to prevent any scratches.

Do not remove these protecting covering while working with Plexiglas.

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As the main body is completed with press board, we can easily measure the top and front cover portions of the flow chamber. Cut the Plexiglas to the required sizes. Here, I have cut the top cover in to two pieces, one covering the air chamber and another for the work area. Do not fix them to the body Remove this covering only when you are satisfied that your work will not cause any damage to the Plexiglas surface.

If you already won a good quality vacuum cleaner, then you will have to spend about Rupees only for the other materials. Please offer your comments and suggestions for any improvement in the above design. Sorry but this is a total waste of time and money. For laminar flow you need the proper hepa filter matched with the proper blower to achieve cfm. Anything else wont proved true lamiar flow.

Also pressurized glove boxes defeat the purpose and increase contaminants.Shroom Supply specializes in mushroom growing supplies, mycology tools, mushroom grow kits, and lab equipment. We put a special emphasis on quality products and customer satisfaction.

Growing edible or medicinal mushrooms is a science. Proper mycology tools and equipment are required for successful results.

diy mycology lab

That's exactly why Shroom Supply exists: To provide both amateur and professional mycologists with high-quality, scientific products and professional-grade mushroom cultivation supplies. We guarantee our products to be amongst the best on the market for growing mushrooms, producing both exceptional results and high yields.

We are always updating our site with new mushroom-related products so please check back often for the latest items and specials. Please take a look at the featured mycology products below and take your time to browse around our website.

Be sure to check out the Categories menu on the left for our complete product selection. We're sure you'll find something! We only ship to the USA and Canada at this time. We ship all USA orders in plain boxes with no indication of the packaged contents. The name "Shroom Supply" will not appear anywhere on the outside of the package. No package will ever have any indication of its contents. We have been in business since Shop with Confidence We have been in business since About Us.

Privacy Notice. Conditions of Use. Contact Us. Discount Coupons.Building a workable mushroom laboratory at home is one of the best things you can do to increase your mushroom growing prowess.

Key skills like making your our grain spawn, doing agar work, cloning fruits, isolating strains, inoculating blocks, and much more are made way easier when you have a sterile environment and a few simple tools.

The basic premise of a mushroom laboratory is to have a space that is clean and free of contamination.

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It can be as simple or as complicated as you want. On the extreme end of the spectrum, you might envision a bright, air-tight and temperature controlled room, complete with white walls, bleached floors, steel counter tops, positive pressure HEPA filtration and full body laboratory suits.

It is simply a large plastic tote, with two holes in front just big enough for your arms. The intention is that the cultivator can do all the necessary lab work inside the box, which can be easily kept clean and relatively draft free. With a still air box, most lab can be done successfully- although contamination rates are higher, and using them is quite cumbersome-especially for anything but very small scale home cultivation.

Most growers find a reasonable solution for a home laboratory somewhere in the middle. The bottom line is that you need to find a solution that works for you, depending on your growing needs, the space you have available, and the amount of money and time you want to invest in your lab. Using a laminar flow hood changes the game completely, and is the cornerstone of a functional home lab. This indispensable tool allows for the home cultivator to set up a semi-permanent and completely functional laboratory at home.

Although many people like to set up and dedicate an entire room of their house for a lab, a flow hood can be easily set up on a table and placed in a bedroom, office, basement or closet. Whatever works for you! As long as there is a clean stream of air coming out of the front of the hood, most mycological work can be done with extremely low rates of contamination. Everything will be made way easier if you have adequate space to work- so your table should be big enough to hold your flow hood, all your tools, uncolonized fruiting blocks and jars, and everything else that you may need.

You want to be able to wipe down the table surface with alcohol before any lab work begins. A simple 4 x 8 sheet of plywood, wrapped in a washable poly sheeting and placed on sawhorses works awesome. You need a way to start, grow out, and store cultures. By far the best way to do this is by using pre-sterilized and disposable petri dishes.

The size I use is mm x 15 mm. They come in 25 dish sleeves, and are sterile as long as the sleeve has not yet been opened. You can get glass dishes that you can use over and over again, but sterilizing these in a pressure cooker is a bit of a pain. You can get these online, but if you have a university nearby, you might want to check with the biological department and see if they sell equipment to the public.

diy mycology lab

You can often get lots of awesome lab supplies, including petri dishes, for very reasonable prices. My personal favourite recipe is Agar-Agar powder I use bacto-agarbarley malt extract and nutritional yeast. You can use a variety of different nutrients potato starch, dog food, ect but the agar agar is key.

Check out this article to learn the process I use for making perfect plates! It actually works reasonably well. This is an essential item for sealing dishes while still allowing them to breathe. Parafilm is a thin waxy plastic film that can be cut into strips and stretched over the edges of plates. It will naturally adhere to the plates when stretched. Not as user friendly, but does the trick in a pinch.

This almost goes without saying, but a good pressure cooker that can hold a reasonable amount of stuff and rated to 15PSI is absolutely essential to having a functional lab. You can use a pressure cooker to sterilize grains, agar, fruiting blocks, syringes… anything that has to be completely clean! You can get as fancy or as simple as you want.

If you are overly enthusiastic about sterilizing, than you may want to consider the All American Electric Sterilizer.I'm a word nerd who loves to geek out on how emerging technology affects the lexicon.

I was an editor on the first 40 volumes of MAKE, and I love shining light on the incredible makers in our community. In particular, covering art is my passion — after all, art is the first thing most of us ever made. When not fawning over perfect word choices, I can be found on the nearest mountain, looking for untouched powder fields and ideal alpine lakes. Mushrooms are fascinating and tastyand this article introduces you to what it takes to make them grow. I love this illustration:.

The project takes about an hour to build and about 2 weeks to grow, and provides a fun and easy window into the magical world of mycelium.

Philip Ross has extensive experience with mushrooms, and incorporates them as well as plant life into his artwork. Other projects in Volume 07 include extracting and replicating your own DNA, hacking your plants through grafting and pollination, freezing and reviving a garden snail, building a videocam rocket and a Stirling engine, and a whole lot more.

The good news is if you subscribeyou can have digital access to all 17 volumes of MAKE! Latest Goli Mohammadi. By Goli Mohammadi Goli Mohammadi. Related Stories from Make:. Send this to a friend Your email Recipient email Send Cancel. Thanks for signing up. Please try again.


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